ND: |
ME11178943 |
PMID: |
11178943 |
LR: |
20061115 |
CED: |
20010222 |
DCO: |
20010419 |
Autoren: |
Johnson KA; Hulse DA; Hart RC; Kochevar D; Chu Q |
Titel: |
Effects of an orally administered mixture of chondroitin sulfate, glucosamine hydrochloride and manganese ascorbate on synovial fluid chondroitin sulfate 3B3 and 7D4 epitope in a canine cruciate ligament transection model of osteoarthritis. |
Quelle: |
Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society; VOL: 9 (1); p. 14-21 /200101/ |
PM: |
Print |
SU: |
IM |
Sprache: |
English |
CY: |
England |
JID: |
9305697 |
ISSN: |
1063-4584 |
Institution: |
Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, Ohio 43210-1089, USA. johnson.2064@osu.edu |
DT: |
Journal Article; Research Support, Non-U.S. Gov't |
Schlagwörter |
CT: |
ADMINISTRATION, ORAL; ANIMALS; CARTILAGE, ARTICULAR/*drug effects; CHONDROITIN SULFATES/administration & dosage; CHONDROITIN SULFATES/*pharmacology; CROSS-SECTIONAL STUDIES; DOGS; FEMALE; GLUCOSAMINE/administration & dosage; GLUCOSAMINE/*pharmacology; GLYCOSAMINOGLYCANS/*metabolism; KNEE JOINT/physiopathology; LIGAMENTS, ARTICULAR/physiopathology; LONGITUDINAL STUDIES; MALE; MANGANESE COMPOUNDS/administration & dosage; MANGANESE COMPOUNDS/*pharmacology; SYNOVIAL FLUID/*chemistry |
CTG: |
APPLIKATION, ORALE; TIER; KNORPEL, GELENK-/*Arzneimittelwirkungen; CHONDROITINSULFATE/Verabreichung & Dosierung; CHONDROITINSULFATE/*Pharmakologie; QUERSCHNITTSSTUDIEN; HUNDE; WEIBLICH; GLUCOSAMIN/Verabreichung & Dosierung; GLUCOSAMIN/*Pharmakologie; GLYCOSAMINO-GLYCANE/*Stoffwechsel; KNIEGELENK/Pathophysiologie; BÄNDER, GELENK-/Pathophysiologie; LONGITUDINALSTUDIEN; MÄNNLICH; MANGANVERBINDUNGEN/Verabreichung & Dosierung; MANGANVERBINDUNGEN/*Pharmakologie; SYNOVIALFLÜSSIGKEIT/*Chemie |
TE: |
Glycosaminoglycans; Manganese Compounds; Glucosamine/3416-24-8; A73025/64082-61-7; Chondroitin Sulfates/9007-28-7 |
CR: |
3416-24-8; 64082-61-7; 9007-28-7 |
AB: |
OBJECTIVE: To evaluate effects of an orally administered mixture of chondroitin sulfate, glucosamine hydrochloride and manganese ascorbate (CS-G-M) on articular cartilage metabolism in dogs with cranial cruciate ligament (CCL) deficient and reconstructed knees, as reflected by concentrations of synovial fluid 3B3, 7D4 and total sulfated glycosaminoglycan (GAG). METHODS: Sixteen adult dogs that underwent unilateral CCL transection were randomized into four groups. Thereafter, group I (N=3) had a sham CCL reconstruction, group II (N=3) had CS-G-M and sham CCL reconstruction, group III (N=5) had CCL reconstruction, and group IV (N=5) had CS-G-M and CCL reconstruction. Synovial fluid collected at 0, 1, 3 and 5 months was examined by ELISA for 3B3 and 7D4 epitope, and by DMMB assay for total GAG. RESULTS: Synovial fluid from CCL transected knees of CS-G-M treated dogs contained significantly elevated concentrations of 3B3 (P=0.029), 7D4 (P=0.036) and 7D4/GAG (P=0.007) in comparison to controls, in a cross-sectional analysis at 3 months. Furthermore, 7D4 and 7D4/GAG concentrations remained significantly elevated (P=0.012) in CCL transected knees of CS-G-M treated dogs over the 5 month period. However, when epitope concentrations were expressed as a ratio of CCL-transected to contralateral non-operated knee, treatment effect of CS-G-M was no longer significant. Reconstruction of the CCL had no significant effect on synovial fluid epitope. CONCLUSIONS: Administration of CS-G-M was associated with altered concentrations of 3B3 and 7D4 epitope in synovial fluid, suggesting that these compounds may act to modulate articular cartilage matrix metabolism in vivo. |