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Suchschritt : FT=glucosamine AND FT=osteoarthritis
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ND: | ME12750890
PMID: | 12750890
LR: | 20061115
CED: | 20030815
DCO: | 20040528
Autoren: | Windhaber RA; Wilkins RJ; Meredith D
Titel: | Functional characterisation of glucose transport in bovine articular chondrocytes.
Quelle: | Pflügers Archiv : European journal of physiology; VOL: 446 (5); p. 572-7 /200308/
PM: | Print-Electronic
EPD: | 20030515
SU: | IM
Sprache: | English
CY: | Germany
JID: | 0154720
ISSN: | 0031-6768
CO: | PFLABK
Institution: | University Laboratory of Physiology, Parks Road, Oxford OX1 3PT, UK.
DT: | In Vitro; Journal Article; Research Support, Non-U.S. Gov't
Schlagwörter
CT: | ANIMALS; ANTIMETABOLITES/pharmacokinetics; BIOLOGICAL TRANSPORT/drug effects; BIOLOGICAL TRANSPORT/physiology; CARTILAGE, ARTICULAR/*cytology; CARTILAGE, ARTICULAR/*metabolism; CATTLE; CHONDROCYTES/*metabolism; DEOXYGLUCOSE/pharmacokinetics; GLUCOSAMINE/pharmacology; GLUCOSE/*metabolism; HYDROGEN-ION CONCENTRATION; HYDROSTATIC PRESSURE; INTERLEUKIN-1/pharmacology; TEMPERATURE
CTG: | TIER; ANTIMETABOLITEN/Pharmakokinetik; BIOLOGISCHER TRANSPORT/Arzneimittelwirkungen; BIOLOGISCHER TRANSPORT/Physiologie; KNORPEL, GELENK-/*Zytologie; KNORPEL, GELENK-/*Stoffwechsel; RINDER; CHONDROZYTEN/*Stoffwechsel; DESOXYGLUCOSE/Pharmakokinetik; GLUCOSAMIN/Pharmakologie; GLUCOSE/*Stoffwechsel; WASSERSTOFFIONENKONZENTRATION; HYDROSTATISCHER DRUCK; INTERLEUKIN-1/Pharmakologie; TEMPERATUR
TE: | Antimetabolites; Interleukin-1; Deoxyglucose/154-17-6; Glucosamine/3416-24-8; Glucose/50-99-7
CR: | 154-17-6; 3416-24-8; 50-99-7
AB: | The adequate provision of glucose to articular chondrocytes is essential to sustain their predominantly anaerobic metabolism; glucose is also a precursor for the extracellular matrix macromolecules which these cells synthesise. Impaired glucose uptake would compromise cell function and potentially result in an imbalance of matrix synthesis and degradation, leading to osteoarthritis. We studied the glucose influx pathway into bovine articular chondrocytes using 2-deoxy- d-[(3)H]-glucose (DOG). Uptake occurs via an extracellular pH (pH(o))-insensitive, phloretin- and cytochalasin B-sensitive pathway, hallmarks of the GLUT family of facilitative glucose transporters, with a K(m) of 0.35+/-0.11 mM. Uptake was affected by a number of physiologically relevant factors: (1) raised hydrostatic pressure (1-30 MPa) inhibited DOG uptake by up to 30%; (2) interleukin-1 (IL-1beta) reduced uptake via an increase in transporter affinity; (3) glucosamine inhibited glucose uptake in a manner consistent with the actions of a competitive inhibitor. Given the involvement of IL-1beta in osteoarthritis and the protective role assigned to glucosamine, these findings implicate an important role for glucose delivery in chondrocyte energy production and matrix metabolism, which, therefore, may potentially affect the maintenance of cartilage integrity.

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