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Suchschritt : FT=glucosamine AND FT=osteoarthritis
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ND: | ME15144122
PMID: | 15144122
LR: | 20061115
CED: | 20040517
DCO: | 20040804
Autoren: | Nakamura H; Shibakawa A; Tanaka M; Kato T; Nishioka K
Titel: | Effects of glucosamine hydrochloride on the production of prostaglandin E2, nitric oxide and metalloproteases by chondrocytes and synoviocytes in osteoarthritis.
Quelle: | Clinical and experimental rheumatology; VOL: 22 (3); p. 293-9 /2004 May-Jun/
PM: | Print
SU: | IM
Sprache: | English
CY: | Italy
JID: | 8308521
ISSN: | 0392-856X
CO: | CERHDP
Institution: | Department of Bioregulation, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan. nakamura@marianna-u.ac.jp
DT: | Journal Article; Research Support, Non-U.S. Gov't
Schlagwörter
CT: | AGED; AGED, 80 AND OVER; CELLS, CULTURED; CHONDROCYTES/*drug effects; CHONDROCYTES/metabolism; CYCLOOXYGENASE 2; DINOPROSTONE/*metabolism; DOSE-RESPONSE RELATIONSHIP, DRUG; GLUCOSAMINE/*pharmacology; HUMANS; INTERLEUKIN-1/pharmacology; ISOENZYMES/genetics; ISOENZYMES/metabolism; MEMBRANE PROTEINS; METALLOPROTEASES/*metabolism; MIDDLE AGED; NITRIC OXIDE/*metabolism; OSTEOARTHRITIS/*metabolism; PROSTAGLANDIN-ENDOPEROXIDE SYNTHASES/genetics; PROSTAGLANDIN-ENDOPEROXIDE SYNTHASES/metabolism; RNA, MESSENGER/genetics; RNA, MESSENGER/metabolism; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SYNOVIAL MEMBRANE/*drug effects; SYNOVIAL MEMBRANE/metabolism
CTG: | ALTE MENSCHEN; ALTE MENSCHEN, 80 JAHRE UND ÄLTER; ZELLEN, KULTIVIERTE; CHONDROZYTEN/*Arzneimittelwirkungen; CHONDROZYTEN/Stoffwechsel; CYCLOOXYGENASE 2; DINOPROSTON/*Stoffwechsel; DOSIS-WIRKUNGSBEZIEHUNG, ARZNEIMITTEL-; GLUCOSAMIN/*Pharmakologie; MENSCH; INTERLEUKIN-1/Pharmakologie; ISOENZYME/Genetik; ISOENZYME/Stoffwechsel; MEMBRANPROTEINE; METALLOPROTEASEN/*Stoffwechsel; MENSCHEN IM MITTLEREN LEBENSALTER; STICKSTOFFMONOXID/*Stoffwechsel; OSTEOARTHROSE/*Stoffwechsel; PROSTAGLANDIN-ENDOPEROXID-SYNTHASE/Genetik; PROSTAGLANDIN-ENDOPEROXID-SYNTHASE/Stoffwechsel; RNA, MESSENGER-/Genetik; RNA, MESSENGER-/Stoffwechsel; REVERSE TRANSKRIPTASE-POLYMERASE-KETTENREAKTION; SYNOVIALMEMBRAN/*Arzneimittelwirkungen; SYNOVIALMEMBRAN/Stoffwechsel
TE: | Interleukin-1; Isoenzymes; Membrane Proteins; RNA, Messenger; Nitric Oxide/10102-43-9; Glucosamine/3416-24-8; Dinoprostone/363-24-6; Cyclooxygenase 2/E.C. 1.14.99.1; PTGS2 protein, human/E.C. 1.14.99.1; Prostaglandin-Endoperoxide Synthases/E.C. 1.14.99.1; Metalloproteases/E.C. 3.4.-
CR: | 10102-43-9; 3416-24-8; 363-24-6; E.C. 1.14.99.1; E.C. 1.14.99.1; E.C. 1.14.99.1; E.C. 3.4.-
AB: | OBJECTIVES: To determine the response of glucosamine hydrochloride on chondrocytes and synoviocytes in terms of prostaglandin E2 (PGE2), nitric oxide (NO) and matrix metalloproteases (MMPs). METHODS: Chondrocytes and synoviocytes were prepared from joint specimens of patients who underwent total knee arthroplasty for osteoarthritis (OA). Chondrocytes from patients with femoral neck fracture were served as a normal control. Culture cells were stimulated by 5 ng/ml of IL-1beta and treated with various concentration of glucosamine hydrochloride (from 1 microg/ml to 500 microg/ml). PGE2, NO, MMP-1, MMP-3, and MMP-13 levels were evaluated in the culture supernatant. Further, the expression of COX-2 mRNA was studied by semiquantitative PCR. RESULTS: With IL-1beta stimulation, the levels of these mediators increased dramatically, except for NO from synoviocytes. After stimulation, levels of these mediators in OA chondrocytes were higher than synoviocytes and normal chondrocytes, and the level of MMP-3 was higher than those of MMP-1 and MMP-13. Glucosamine hydrochloride at a concentration of 100 microg/ml suppressed PGE2 production, and partly suppressed NO production. It also suppressed the production of MMPs from normal chondrocytes and synoviocytes but not from OA chondrocytes. CONCLUSION: Glucosamine modulates the metabolism of chondrocytes and synoviocytes and its mode of action differs between cells and conditions.

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