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Suchschritt : FT=glucosamine AND FT=osteoarthritis
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2/379 von 416 DIMDI: MEDLINE (ME60) © NLM

ND: | ME03922432
PMID: | 3922432
LR: | 20061115
CED: | 19850722
DCO: | 19850722
Autoren: | Cox MJ; McDevitt CA; Arnoczky SP; Warren RF
Titel: | Changes in the chondroitin sulfate-rich region of articular cartilage proteoglycans in experimental osteoarthritis.
Quelle: | Biochimica et biophysica acta; VOL: 840 (2); p. 228-34 /19850618/
PM: | Print
SU: | IM
Sprache: | English
CY: | NETHERLANDS
JID: | 0217513
ISSN: | 0006-3002
CO: | BBACAQ
DT: | Journal Article; Research Support, Non-U.S. Gov't
Schlagwörter
CT: | AMINO SUGARS/analysis; ANIMALS; CARTILAGE, ARTICULAR/*metabolism; CENTRIFUGATION, ISOPYCNIC; CHEMISTRY; CHONDROITIN/*analogs & derivatives; CHONDROITIN SULFATES/isolation & purification; CHONDROITIN SULFATES/*metabolism; DOGS; HYDROLYSIS; HYDROXYLAMINES; OSTEOARTHRITIS/*metabolism; PROTEOGLYCANS/isolation & purification; PROTEOGLYCANS/*metabolism
CTG: | AMINOZUCKER/Analyse; TIER; KNORPEL, GELENK-/*Stoffwechsel; ZENTRIFUGATION, ISOPYKNISCHE; CHEMIE; CHONDROITIN/*Analoge & Derivate; CHONDROITINSULFATE/Isolierung & Reinigung; CHONDROITINSULFATE/*Stoffwechsel; HUNDE; HYDROLYSE; HYDROXYLAMINE; OSTEOARTHROSE/*Stoffwechsel; PROTEOGLYCANE/Isolierung & Reinigung; PROTEOGLYCANE/*Stoffwechsel
TE: | Amino Sugars; Hydroxylamines; Proteoglycans; Chondroitin/9007-27-6; Chondroitin Sulfates/9007-28-7
CR: | 9007-27-6; 9007-28-7
AB: | The chondroitin sulfate-rich region was cleaved from cartilage proteoglycans of experimental osteoarthritic canine joints to establish whether changes in this region of the molecule contribute to the well-documented increase in the chondroitin sulfate to keratan sulfate ratio in osteoarthritis. Experimental osteoarthritis was induced in eight dogs by severance of the right anterior cruciate ligament, the left joint serving as a control. Proteoglycans were extracted from the femoral cartilage of both joints, isolated as A1 fractions by associative density gradient centrifugation and cleaved with hydroxylamine. The chondroitin sulfate-rich region was isolated by either gel chromatography or dissociative density gradient centrifugation. The chondroitin sulfate-rich region from the proteoglycans of the experimental osteoarthritic joints was slightly larger in hydrodynamic size and had both a higher uronate/protein weight ratio and galactosamine/glucosamine molar ratio than the corresponding control. We conclude that the chondroitin sulfate-rich region of proteoglycans in articular cartilage of experimental osteoarthritic joints is larger and has more chondroitin sulfate than that of proteoglycans of normal cartilage.

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