Dokumente
Suchschritt : FT=glucosamine AND FT=osteoarthritis
» Fenster schließen »

2/99 von 416 DIMDI: MEDLINE (ME60) © NLM

ND: | ME15993867
PMID: | 15993867
LR: | 20061115
CED: | 20050729
DCO: | 20051018
Autoren: | Brockhausen I; Carran J; McEleney K; Lehotay M; Yang X; Yin L; Anastassiades T
Titel: | N-Acyl derivatives of glucosamine as acceptor substrates for galactosyltransferase from bone and cartilage cells.
Quelle: | Carbohydrate research; VOL: 340 (12); p. 1997-2003 /20050905/
PM: | Print
SU: | IM
Sprache: | English
CY: | Netherlands
JID: | 0043535
ISSN: | 0008-6215
CO: | CRBRAT
Institution: | Department of Medicine, The Arthritis Centre, Queen's University, Kingston General Hospital, Kingston, Ontario, Canada K7L 2V7. brockhau@post.queensu.ca
DT: | Journal Article; Research Support, Non-U.S. Gov't
Schlagwörter
CT: | ANIMALS; CATTLE; CELL PROLIFERATION/drug effects; CELLS, CULTURED; CHONDROCYTES/drug effects; CHONDROCYTES/*enzymology; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GLUCOSAMINE/*analogs & derivatives; GLUCOSAMINE/*metabolism; GLUCOSAMINE/pharmacology; HUMANS; MILK/enzymology; N-ACETYLLACTOSAMINE SYNTHASE/*metabolism; OSTEOBLASTS/*enzymology; OSTEOSARCOMA/enzymology
CTG: | TIER; RINDER; ZELLPROLIFERATION/Arzneimittelwirkungen; ZELLEN, KULTIVIERTE; CHONDROZYTEN/Arzneimittelwirkungen; CHONDROZYTEN/*Enzymologie; CHROMATOGRAPHIE, HOCHDRUCK-FLÜSSIGKEITS-; GLUCOSAMIN/*Analoge & Derivate; GLUCOSAMIN/*Stoffwechsel; GLUCOSAMIN/Pharmakologie; MENSCH; MILCH/Enzymologie; N-ACETYLLACTOSAMIN-SYNTHASE/*Stoffwechsel; OSTEOBLASTEN/*Enzymologie; OSTEOSARKOM/Enzymologie
TE: | N-butyrylglucosamine; Glucosamine/3416-24-8; N-Acetyllactosamine Synthase/E.C. 2.4.1.90
CR: | 3416-24-8; E.C. 2.4.1.90
AB: | Glucosamine is commonly used as a nutraceutical by arthritis patients. However, its mode of action is still unknown, and there is controversy about its clinical efficacy. Synthetic N-acyl glucosamines (acyl group>2 carbons) comprise a new class of drugs. We examined these derivatives for their effect in bone and cartilage cells, and for their ability to serve as acceptor substrates for galactosyltransferase. With the exception of N-benzoylglucosamine, compounds of the series were good substrates for galactosyltransferases from bone and cartilage cells, and for purified enzyme from bovine milk. When N-butyrylglucosamine (GlcNBu) was added to the cell medium of primary bovine chondrocytes and human osteoblasts, small amounts were found to enter the cells and a radiolabeled metabolite appeared in the medium. However, GlcNBu did not appear to be incorporated directly into oligosaccharides. GlcNBu at 1 and 5mM concentrations in the glucose-free cell medium of primary human osteoblasts from osteoarthritis patients did not significantly alter cell proliferation or cell differentiation.

» Volltext »
» Fenster schließen »