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Suchschritt : FT=glucosamine AND FT=osteoarthritis
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2/316 von 416 DIMDI: MEDLINE (ME60) © NLM

ND: | ME10806048
PMID: | 10806048
LR: | 20061115
CED: | 20000803
DCO: | 20000803
Autoren: | Piperno M; Reboul P; Hellio Le Graverand MP; Peschard MJ; Annefeld M; Richard M; Vignon E
Titel: | Glucosamine sulfate modulates dysregulated activities of human osteoarthritic chondrocytes in vitro.
Quelle: | Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society; VOL: 8 (3); p. 207-12 /200005/
PM: | Print
SU: | IM
Sprache: | English
CY: | ENGLAND
JID: | 9305697
ISSN: | 1063-4584
Institution: | Centre Hospitalier Lyon Sud, Claude Bernard University, 165 chemin du Grand Revoyet, Pierre Bénite, France.
DT: | Journal Article; Research Support, Non-U.S. Gov't
Schlagwörter
CT: | CASEINS/drug effects; CELLS, CULTURED; CHONDROCYTES/*drug effects; COLLAGENASES/drug effects; CULTURE MEDIA; CYCLIC AMP/metabolism; DOSE-RESPONSE RELATIONSHIP, DRUG; GLUCOSAMINE/*pharmacology; HUMANS; NITRIC OXIDE/metabolism; OSTEOARTHRITIS/*metabolism; PHOSPHOLIPASES A/drug effects; PROTEIN BIOSYNTHESIS; PROTEIN KINASE C/drug effects
CTG: | CASEINE/Arzneimittelwirkungen; ZELLEN, KULTIVIERTE; CHONDROZYTEN/*Arzneimittelwirkungen; KOLLAGENASEN/Arzneimittelwirkungen; KULTURMEDIEN; CYCLO-AMP/Stoffwechsel; DOSIS-WIRKUNGSBEZIEHUNG, ARZNEIMITTEL-; GLUCOSAMIN/*Pharmakologie; MENSCH; STICKSTOFFMONOXID/Stoffwechsel; OSTEOARTHROSE/*Stoffwechsel; PHOSPHOLIPASEN A/Arzneimittelwirkungen; PROTEINBIOSYNTHESE; PROTEIN-KINASE C/Arzneimittelwirkungen
TE: | Caseins; Culture Media; Nitric Oxide/10102-43-9; Glucosamine/3416-24-8; Cyclic AMP/60-92-4; Protein Kinase C/E.C. 2.7.1.37; Phospholipases A/E.C. 3.1.1.-; Collagenases/E.C. 3.4.24.-
CR: | 10102-43-9; 3416-24-8; 60-92-4; E.C. 2.7.1.37; E.C. 3.1.1.-; E.C. 3.4.24.-
AB: | OBJECTIVE: The efficacy of glucosamine sulfate (GS) in the symptomatic treatment of patients with osteoarthritis (OA) is suggested to be mediated by still unknown effects on the altered OA cartilage. DESIGN: Using human OA chondrocytes in culture, the effects of GS on protein synthesis, caseinase, collagenase, phospholipase A2 (PLA2) and protein kinase C (PKC) activities as well as production of nitric oxide and cyclic AMP were studied in both cells and culture medium. RESULTS: GS significantly reduced PLA2 activity, and more modestly collagenase activity, in the OA chondrocytes in a dose-dependent manner. By contrast, PLA2 and collagenase activity of the culture medium was not modified. No effects on caseinase activity was seen. GS significantly and dose-dependently increased protein synthesis. GS did not modify nitric oxide and cAMP production but significantly increased PKC production. CONCLUSION: GS modified cultured OA chondrocyte metabolism by acting on PKC, cellular PLA2, protein synthesis and possibly collagenase activation. Extrapolation of the effect to the in-vivo situation remains hypothetical but they might represent some possible mechanisms of action of the drug in human.

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