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ND: | ME16504489
PMID: | 16504489
LR: | 20061115
CED: | 20060313
DCO: | 20060510
Autoren: | Qu CJ; Karjalainen HM; Helminen HJ; Lammi MJ
Titel: | The lack of effect of glucosamine sulphate on aggrecan mRNA expression and (35)S-sulphate incorporation in bovine primary chondrocytes.
Quelle: | Biochimica et biophysica acta; VOL: 1762 (4); p. 453-9 /200604/
PM: | Print-Electronic
EPD: | 20060210
SU: | IM
Sprache: | English
CY: | Netherlands
JID: | 0217513
ISSN: | 0006-3002
CO: | BBACAQ
Institution: | Department of Anatomy, Institute of Biomedicine, University of Kuopio, PO Box 1627, 70211 Kuopio, Finland.
DT: | Journal Article; Research Support, Non-U.S. Gov't
Schlagwörter
CT: | AGGRECANS; ANIMALS; CATTLE; CELLS, CULTURED; CHONDROCYTES/*metabolism; EXTRACELLULAR MATRIX PROTEINS/*biosynthesis; EXTRACELLULAR MATRIX PROTEINS/genetics; GENE EXPRESSION REGULATION/drug effects; GLUCOSAMINE/*pharmacology; GLUCURONOSYLTRANSFERASE/biosynthesis; GLYCOSAMINOGLYCANS/biosynthesis; HEXOSAMINES/pharmacology; LECTINS, C-TYPE/*biosynthesis; LECTINS, C-TYPE/genetics; PROTEOCHONDROITIN SULFATES/*biosynthesis; PROTEOCHONDROITIN SULFATES/genetics; RNA, MESSENGER/*biosynthesis; SULFUR RADIOISOTOPES
CTG: | AGGREKANE; TIER; RINDER; ZELLEN, KULTIVIERTE; CHONDROZYTEN/*Stoffwechsel; EXTRAZELLULÄRE MATRIXPROTEINE/*Biosynthese; EXTRAZELLULÄRE MATRIXPROTEINE/Genetik; GENEXPRESSIONSREGULATION/Arzneimittelwirkungen; GLUCOSAMIN/*Pharmakologie; GLUCURONOSYLTRANSFERASE/Biosynthese; GLYCOSAMINO-GLYCANE/Biosynthese; HEXOSAMINE/Pharmakologie; LECTINE, C-TYP-/*Biosynthese; LECTINE, C-TYP-/Genetik; PROTEOCHONDROITIN-SULFATE/*Biosynthese; PROTEOCHONDROITIN-SULFATE/Genetik; RNA, MESSENGER-/*Biosynthese; SCHWEFELRADIOISOTOPE
TE: | Aggrecans; Extracellular Matrix Proteins; Glycosaminoglycans; Hexosamines; Lectins, C-Type; Proteochondroitin Sulfates; RNA, Messenger; Sulfur Radioisotopes; Glucosamine/3416-24-8; Glucuronosyltransferase/E.C. 2.4.1.17; hyaluronan synthase/E.C. 2.4.1.212
CR: | 3416-24-8; E.C. 2.4.1.17; E.C. 2.4.1.212
AB: | Glucosamine and glucosamine sulphate have been promoted as a disease-modifying agent to improve the clinical symptoms of osteoarthritis. The precise mechanism of the action of the suggested positive effect of glucosamine or glucosamine sulphate on cartilage proteoglycans is not known, since the level of glucosamine in plasma remains very low after oral administration of glucosamine sulphate. We examined whether exogenous hexosamines or their sulphated forms would increase steady-state levels of aggrecan and hyaluronan synthase (HAS) or glycosaminoglycan synthesis using Northern blot and (35)S-sulphate incorporation analyses. Total RNA was extracted from bovine primary chondrocytes which were cultured either in 1 mM concentration of glucosamine, galactosamine, mannosamine, glucosamine 3-sulphate, glucosamine 6-sulphate or galactosamine 6-sulphate for 0, 4, 8 and 24 h, or in three different concentrations (control, 100 microM and 1 mM) of glucosamine sulphate salt or glucose for 24 or 72 h. Northern blot assay showed that neither hexosamines nor glucosamine sulphate salt stimulated aggrecan and HAS-2 mRNA expression. Glycosaminoglycan synthesis remained at a control level in the treated cultures, with the exception of mannosamine which inhibited (35)S-sulphate incorporation in low-glucose DMEM treatment. In our culture conditions, hexosamines or their sulphated forms did not increase aggrecan expression or (35)S-sulphate incorporation.

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