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Suchschritt : FT=glucosamine AND FT=osteoarthritis
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2/381 von 416 DIMDI: MEDLINE (ME60) © NLM

ND: | ME06691859
PMID: | 6691859
LR: | 20061115
CED: | 19840220
DCO: | 19840220
Autoren: | Ryu J; Treadwell BV; Mankin HJ
Titel: | Biochemical and metabolic abnormalities in normal and osteoarthritic human articular cartilage.
Quelle: | Arthritis and rheumatism; VOL: 27 (1); p. 49-57 /198401/
PM: | Print
SU: | AIM IM
Sprache: | English
CY: | UNITED STATES
JID: | 0370605
ISSN: | 0004-3591
CO: | ARHEAW
DT: | Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.
NG: | AM16265 AM NIADDK
Schlagwörter
CT: | CARBON RADIOISOTOPES/diagnostic use; CARTILAGE, ARTICULAR/*metabolism; FEMALE; HUMANS; HYALURONIC ACID/biosynthesis; MALE; OSTEOARTHRITIS/*metabolism; PROTEOGLYCANS/biosynthesis; SULFUR RADIOISOTOPES/diagnostic use; TRITIUM/diagnostic use
CTG: | KOHLENSTOFFRADIOISOTOPE/diagnostische Verwendung; KNORPEL, GELENK-/*Stoffwechsel; WEIBLICH; MENSCH; HYALURONSÄURE/Biosynthese; MÄNNLICH; OSTEOARTHROSE/*Stoffwechsel; PROTEOGLYCANE/Biosynthese; SCHWEFELRADIOISOTOPE/diagnostische Verwendung; TRITIUM/diagnostische Verwendung
TE: | Carbon Radioisotopes; Proteoglycans; Sulfur Radioisotopes; Tritium/10028-17-8; Hyaluronic Acid/9004-61-9
CR: | 10028-17-8; 9004-61-9
AB: | Incorporation of radioactive precursors into macromolecules was studied with human normal and osteoarthritic articular cartilage organ culture. Analysis of the salt extracted matrix components separated by cesium chloride buoyant density gradient centrifugation showed an increase in the specific activities of all gradient fractions prepared from the osteoarthritic cartilage. Further analysis of these fractions showed the osteoarthritic cartilage contained 5 times as much sulfate incorporated into proteoglycans, and an even greater amount of 3H-glucosamine incorporated into material sedimenting to the middle of the gradient. Greater than half of this radioactive middle fraction appears to be hyaluronate, as judged by the position it elutes from a DEAE column and its susceptibility to hyaluronidase digestion. This study supports earlier findings showing increased rates of macromolecular synthesis in osteoarthritis, and in addition, an even greater synthetic rate for hyaluronic acid is demonstrated.

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